Treatment of C. difficile toxin B associated conditions

ABSTRACT

This invention relates to prevention and/or treatment of antibiotic associated diarrhea, including Clostridium difficile associated diarrhea (CDAD), pseudomembranous colitis (PMC) and other conditions associated with C. difficile infection, using oligosaccharide compositions which bind C. difficile toxin B. More specifically, the invention concerns neutralization of C. difficile toxin B associated with such conditions.

This application is a continuation of application Ser. No. 09/085,032,filed May 28, 1998 now U.S. Pat. No. 6,013,635.

FIELD OF THE INVENTION

This invention relates to treatment of antibiotic associated diarrhea,including Clostridium difficile associated diarrhea (CDAD) andpseudomembranous colitis (PMC) and other conditions associated with C.difficile infection. More specifically, the invention concernsneutralization of C. difficile toxin B, a cytotoxin associated withCDAD, PMC and other conditions caused by C. difficile.

REFERENCES

The following references are cited in the application as numbers inbrackets ([]) at the relevant portion of the application.

1. Bartlett, J. G., et al., "Antibiotic-associated pseudomembranouscolitis due to toxin-producing clostridia", N. Engl. J. Med.,298:531-534 (1978).

2. Lyerly, D. M., "Epidemiology of Clostridium difficile disease", Clin.Microbiol. News, 15:49-53 (1993).

3. Cozart, J. C., et al., "Clostridium difficile diarrhea in patientswith AIDS versus non-AIDS controls. Method of treatment and clinicalresponse to treatment", J. Clin. GastroenteroL, 16:19-24 (1993).

4. Barbut, F., et al., "Comparison of Enterotoxin Production, cytotoxinproduction, serogrouping and antimicrobial susceptibilities ofClostridium difficile strains isolated from AIDS and humanimmunodeficiency virus-negative patients", J. Clin. Microbiol., 31:740-2(1993).

5. Krivan, H. C., et al., "Cell surface binding site for Clostridiumdifficile enterotoxin: evidence for a glycoconjugate containing thesequence αGal(1-3)βGal(1-4)βGlcNAc", Infect Immun., 53:573-81(1986).

6. Clark, G. F., et al., "Toxin A from Clostridium difficile binds torabbit erythrocyte glycolipids with terminal αGal(1-3)βGal(1-4)βGlcNAcsequences", Arch. Biochem. Biophys., 257:217-29 (1987).

7. Tucker, K. D., et al., "Toxin A of Clostridium difficile binds tocarbohydrate antigens I, X, and Y", Infect. Immun., 59:73-8 (1991).

8. Krivan, H. C., et al., "Purification of Clostridium difficile toxin Aby affinity chromatography on immobilized bovine thyroglobulin", Infect.Immun., 55:1873-7 (1987).

9. Kamiya, S., et al., "Analysis of purity of Clostridium difficiletoxin A derived by affinity chromatography on immobilized bovinethyroglobulin", FEMS Microbiol. Lett., 56:331-6 (1988).

10. Armstrong, G. D., et al., "Investigation of shiga-like toxin bindingto chemically synthesized oligosaccharide sequences", J. Infect. Dis.,164:1160-7 (1991).

11. Von Eichel-Streiber, C., et al., "Clostridium difficile toxin Acarries a c-terminal repetitive structure homologous to the carbohydratebinding region of streptococcal glycosyltransferases", Gene, 96:107-13(1990).

12. Lemieux, R. U., et al., "The properties of a `synthetic` antigenrelated to the blood-group Lewis A", J. Am. Chem. Soc., 97:4076-83(1975).

13. Sullivan, N. M., et al., "Purification and characterization of toxinA and B from Clostridium difficile", Infect. Immun., 35:1032-40 (1983).

14. Finegold, S. M., et al., "Therapy directed against Clostridiumdifficile and its toxins. Complications of therapy". In Rolfe, R.D.,etal., (eds) C. difficile: It's Role in Intestinal Disease, AcademicPress, Inc., San Diego, Calif., 341-57 (1988).

15. Bartlett, J. G., et al., "Symptomatic relapse after oral vancomycintherapy of antibiotic-associated pseudomembranous colitis",Gastroenterology, 78:431-4 (1989).

16. Tedesco, F. J., "Pseudomembranous colitis: Pathogenesis andtherapy", Med. Clin. North Am., 66:655-64 (1982).

17. Keighley, M. R. B., "Antibiotic-associated pseudomembranous colitis:pathogenesis and management", Drugs, 20:449-56 (1980).

18. Bartlett, J. D., "Treatment of antibiotic-associatedpseudomembranous colitis", Rev. Infect. Dis., 6, Suppl. 1:1-55 (1984).

19. Onderdonk, A. B., et al., "Comparative effects of clindamycin andclindamycin metabolites in the hamster model for antibiotic-associatedcolitis", J. Antimicrob. Chem., 8:383-93 (1981).

20. Triadfilopoulos, G., et al., "Differential effects of Clostridiumdifficile toxin a and b on rabbit ileum", Gastroenterology, 93:273-9(1987).

21. Lemieux, R. U., et al., "Glycoside-Ether-Ester Compounds", U.S. Pat.No. 4,137,401, issued Jan. 30, 1979.

22. Lemieux, R. U., et al., "Artificial Oligosaccharide AntigenicDeterminants", U.S. Pat. No. 4,238,473, issued Dec. 9, 1980.

23. Lemieux, R. U., et al., "Synthesis of 2-Amino-2-Deoxyglycoses and2-Amino-2-Deoxyglycosides from glycals", U.S. Pat. No. 4,362,720, issuedDec. 7, 1982.

24. Cox, D., et al. "A New Synthesis of4-0-α-D-Galactopyranosyl-D-Galacto-Pyranose", Carbohy. Res., 62: 245-252(1978).

25. Dahmen, J., et al., "Synthesis of space arm, lipid, and ethylglycosides of the trisaccharide portion[α-D-Gal-(1-4)-β-D-Gal(1-4)-β-D-Glc] of the blood group p^(k) antigen:preparation of neoglycoproteins", Carbohy. Res., 127:15-25 (1984).

26. Garegg, P. J., et al., "A Synthesis of 8-Methoxycarbonyloct-1-ylO-α-D-Galactopyranosyl-(1→3)-0-β-D-Galactopyranosyl-(1-.fwdarw.4)-2-Acetamido-2-Deoxy-β-D-Glucopyranoside",Carbohy. Res., 136: 207-213 (1985).

27. Garegg, P. J., et al., "Synthesis of 6- and 6'-deoxy derivatives ofmethyl 4-0-α-D-galactopyranosyl-β-D-galactopyranoside for studies ofinhibition of pyelonephritogenic fimbriated E. coli adhesion to urinaryepithelium-cell surfaces", Carbohy. Res., 137: 270-275 (1985).

28. Jacquinet, J. C., et al., "Synthesis of Blood-group Substances, Part11. Synthesis of the TrisaccharideO-α-D-Galactopyranosyl-(1→3)-O-β-D-galactopyranosyl-(1.fwdarw.4)-2-acetamido-2-deoxy-D-glucopyranose",J. C. S. Perkin, 1:326-330 (1981).

29. Koike, K., et al., "Total Synthesis of Globotriaosyl-E andZ-Ceramides and lsoglobotriaosyl-E-Ceramide," Carbohy. Res., 163:189-208 (1987).

30. Schaubach, R., et al., "Tumor-Associated Antigen Synthesis:Synthesis of the Gal-α-(1→3)-Gal-β-(1→4)-GlCNAc Epitope. A specificDeterminant for Metastatic Progression?," Liebigs Ann. Chem., 607-614(1991).

31. Ratcliffe, R. M., et al., "Sialic Acid Glycosides, Antigens,Immunoadsorbents, and Methods for Their Preparation", U.S. Pat. No.5,079,353, issued Jan. 7,1992.

32. Okamoto, K., et al., "Glycosidation of Sialic Acid," Tetrahedron,47: 5835-5857 (1990).

33. Abbas, S. A., et al., "Tumor-Associated Oligosaccharide 1: Synthesisof Sialyl-Lewis^(a) Antigenic Determinant", Sialic Acids, Proc.Japan-German Symp. Berlin, 22-23 (1988).

34. Paulsen, "Advances in Selective Chemical Syntheses of ComplexOligosaccharide", Angew. Chem. Int. Ed. Eng., 21:155-173 (1982).

35. Schmidt, "New Methods for the Synthesis of Glycosides andOligosaccharide - Are There Alternatives to the Koenigs-Knorr Method?"Angew. Chem. Int Ed. Eng., 25:212-235 (1986).

36. Fugedi, P., et al., "Thioglycosides as Glycosylating Agents inOligosaccharide Synthesis", Glycoconjugate J., 4:97-108 (1987).

37. Kameyama, A., et al., "Total synthesis of sialyl Lewis X", Carbohy.Res., 209: c1-c4 (1991).

38. Ekborg, G., et al., "Synthesis of Three Disaccharides for thePreparation of Immunogens bearing Immunodeterminants Known to Occur onGlycoproteins", Carbohy. Res., 110: 55-67 (1982).

39. Dahmen, J., et al., "2-Bromoethyl glycosides: applications in thesynthesis of spacer-arm glycosides,"Carbohy. Res., 118: 292-301 (1983).

40. Rana, S. S., et al., "Synthesis of Phenyl2-Acetamido-2-Deoxy-3-O-αL-Fucopyranosyl-β-D-Glucopyranoside and RelatedCompounds", Carbohy. Res., 91: 149-157 (1981).

41. Amvam-Zollo, P., et al., "Streptococcus pneumoniae Type XIVPolysaccharide: Synthesis of a Repeating Branched Tetrasaccharide withDioxa-Type Spacer-Arms", Carbohy. Res., 150:199-212 (1986).

42. Paulsen, H., "Synthese von oligosaccharid-determinanten mitamid-spacer vom typ des T-antigens", Carbohy. Res., 104:195-219 (1982).

43. Chernyak, A. Y., et al., "A New Type of Carbohydrate-ContainingSynthetic Antigen: Synthesis of Carbohydrate-Containing PolyacrylamideCopolymers having the Specificity of 0:3 and 0:4 Factors of Salmonella",Carbohy. Res., 128: 269-282 (1984).

44. Fernandez-Santana, V., et al., "Glycosides of Monoallyl DiethyleneGlycol. A New type of Spacer group for Synthetic Oligosaccharide", J.Carbohy. Chem., 8(3), 531-537 (1989).

45. Lee, R. T., et al., "Synthesis of 3-(2-Aminoethylthio)PropylGlycosides", Carbohy. Res., 37: 193-201 (1974).

46. Riegler, M., Sedivy, R., Pothoulakis, C., Hamilton, G., Zacherl, J.,Bischof, G., Cosentini, E., Feil, W., Schiessel, R., LaMont, J. T.,Wenzl, E., J. Clin. Invest., 95:2004-2011 (1995).

47. Chang T. W., Gorbach, S. L., Bartlett, J. G., "Inhibition of bindingof Clostridium difficile toxin by steroids", J. Infect. Dis., 142:113(1980).

48. Lyerly, D. M., Krivan, H. C., Wilkens, T. D., "Clostridiumdifficile: Its disease and toxins", Clin. Microb. Rev., 1:1-18 (1988).

49. Wren, B. W., Russell, R. R. and Tabaqchali, S., "Antigeniccross-reactivity and functional inhibition by antibodies to Clostridiumdifficile toxin A, Streptococcus mutans glucan-binding protein, and asynthetic peptide", Infect. Immun., 59:3151-5 (1991).

50. Wren, B. W., "A family of clostridial and streptococcalligand-binding proteins with conserved C-terminal repeat sequences",Mol. Microbiol, 5:797-803 (1991).

51. von Eichel-Streiber, C., Laufenberg-Feldmann, R., Sartingen, S.,Schulze, J., Sauerborn, M., "Comparative sequence analysis of theClostridium difficile toxins A and B", Mol. Gen. Genet., 233:260-268(1992).

52. Smith, D. J., Akita, H., King, W. F., Taubman, M. A., "Purificationand antigenicity of a novel glucan-binding protein of Streptococcusmutans,"Infect. Immun., 62:2545-2552 (1994).

53. Rolfe, R. D., "Binding kinetics of Clostridium difficile toxin A andB to intestinal brush border membranes from infant and adulthamsters,"Infect Immun., 59:1223-1230 (1991).

54. Bartlett, J. G., "Clostridium difficile: history of its role as anenteric pathogen and the current state of knowledge about the organism",Clin. Infect. Dis., 18 (Suppl 4):S265-272 (1994).

55. Bartlett, J. G., Chang, T. W., Gurwith, M., Gorbach, S. L.,Onderdonk, A. B., "Antibiotic-associated pseudomembranous colitis due totoxin-producing clostridia," N. Eng. J. Med. , 298: 531-534 (1978).

56. Triadafilopoulos, G., Pothoulakis, C., O'Brien, M. J., LaMont, J.T., "Differential effects of Clostridium difficile toxins A and B onrabbit ileum," Gastroenterology, 93:273-279 (1987).

57. Lyerly, D. M., Saum, K. E., MacDonald, D. K., Wilkins, T. D.,"Effects of Clostridium difficile toxins given intragastrically toanimals," Infect Immun., 47:349-352 (1985).

58. Torres, J., Jennische, E., Lange, S., Lonnroth, I., "Enterotoxinsfrom Clostridium difficile; diarrhoeogenic potency and morphologicaleffects in the rat intestine," Gut, 31:781-785 (1990).

59. Flegel, W. A., Muller, F., Daubener, W., Fischer, H. G., Hadding,U., Northoff, H., "Cytokine response by human monocytes to Clostridiumdifficile toxin A and toxin B," Infect. Immun., 59:3659-3666 (1991).

60. Heerze, L. D., Kelm, M. A., Talbot, J. A., Armstrong, G. D.,"Oligosaccharide sequences attached to an inert support (SYNSORB) aspotential therapy for antibiotic-associated diarrhea andpseudomembranous colitis," J. Infect. Dis., 169:1291-1296 (1994).

61. Sullivan, N. M., Pellet, S. and Wilkins, T. D., "Purification andcharacterization of toxin A and B from Clostridium difficile," InfectImmun., 35:1032-1040 (1982).

62. Lima, A. A., Lyerly, D. M., Wilkins, T. D., lnnes, D. J., Guerrant,R. L., "Effects of Clostridium difficile toxins A and B in rabbit smalland large intestine in vivo and on cultured cells in vitro," Infect.Immun., 56:582-588 (1988).

63. Donta, S. T., Sullivan, N., Wilkins, T. D., "Differential effects ofClostridium difficile toxins on tissue-cultured cells," J. Clin.Microb., 15:1157-1158 (1982).

64. Fiorentini, C., Thelestam, M., "Clostridium difficile toxin A andits effect on cells," Toxicon, 29:543-567 (1991).

65. Heerze, et al., U.S. Pat. No. 5,484,773 (1996).

66. Heerze, et al., U.S. Pat. No. 5,635,606 (1996).

67. Hinsgaul, O., et al., PCT/CA97/00862 (1997).

68. Hinsgaul, O., et al., PCT/CA97/00863 (1997).

69. Hinsgaul, O., et al., PCT/CA97/00864 (1997).

70. Hinsgaul, et al., PCT/CA97/00851 (1997).

The disclosure of the above publications, patents and patentapplications are herein incorporated by reference in their entirety tothe same extent as if the language of each individual publication,patent and patent application were specifically and individuallyincluded herein.

BACKGROUND OF THE INVENTION

The anaerobic organism Clostridium difficile is the major causativeagent of antibiotic-associated bacterial diarrhea and pseudomembranouscolitis (PMC) among mainly elderly patients in hospitals and long termcare facilities [1,2]. The organism cannot compete successfully with thenormal microbial flora in the adult colon, but when the normalintestinal microflora is altered, for example by antibiotic treatment,C. difficile is able to colonize the gut in high numbers. Antibiotictherapy accounts for 98% of all cases of C. difficile associateddiarrhea (CDAD). However, any predisposing condition which alters thenormal intestinal flora, including any condition which requiresextensive immunosuppressive treatment, can also lead to the developmentof CDAD. For example, recent evidence suggests that AIDS patients arealso high risk candidates for acquiring CDAD [3,4].

C. difficile produces two exotoxins, toxin A (an enterotoxin) and toxinB (a cytotoxin) which appear to play important roles in causing CDAD. Ithas long been thought that toxin A is primarily responsible for thedisease. It acts by binding to epithelial cells in the intestine,resulting in the destruction of these cells and causing the secretion offluid into the intestine. The destruction of these protective epithelialcells by toxin A represents the crucial step leading to the developmentof diarrhea. Once damage has occurred to the epithelial cells, thepotent cytotoxin B can then gain access to underlying sensitive tissuesand initiate additional clinical symptoms[-10,13,19-20,53-56,57-59,61-64]. However, in a recent in vitro study[46], toxin B was found to be more potent at damaging human colonicepithelium than toxin A, suggesting that toxin B may play a moreimportant role in CDAD than previously believed.

Toxin A has been found to display a lectin-like activity which allows itto bind to an oligosaccharide receptor on epithelial cells. Severaloligosaccharide sequences have been identified as potential receptorsfor toxin A [60,66]. The cellular receptor for toxin B has not beendetermined, but there are some indications that toxin B binds toerythrocytes implying that a carbohydrate receptor may be involved intoxin B binding [47, 48]. Steroids have also been proposed as potentialreceptors for toxin B [47].

The current therapy for patients who suffer from CDAD or PMC is toremove the offending drug and begin oral administration of theantibiotics Metronidazole or Vancomycin along with fluid replacement[3,14]. Vancomycin is only used in certain situations when patientscannot tolerate or are not responsive to Metronidazole treatment.Vancomycin is not used routinely because of its high cost and thepossibility that its overuse may encourage the development of Vancomycinresistant microorganisms. Metronidazole therapy is effective in about80% of the patients who suffer from CDAD or PMC. In about 20% ofpatients, the diarrhea returns after discontinuing antibiotic treatment[15]. In such individuals, episodes continue to recur until the normalintestinal flora is reestablished and the number of C. difficileorganisms is reduced. This is a slow process, since antibiotics such asMetronidazole, which disturb the balance of the normal intestinal flora,are administered each time the diarrhea occurs.

The only other treatment for CDAD and PMC which removes toxin activityfrom the intestinal tract involves the use of multigram quantities ofanion exchange resins such as cholestyramine and colestipol given orallyin combination with antibiotics. This approach has been used to treatmild to moderately ill patients, as well as individuals who suffer frommultiple episodes of diarrhea [16,17]. This form of therapy has onlybeen moderately successful in treating the disease [18]. In addition totheir lack of efficacy, there are several other disadvantages associatedwith the use of ion exchange resins. Ion exchange resins do not bindspecifically to toxin A or toxin B. Thus, ion exchange resins may alsobind antibiotics, resulting in suboptimal levels of antibiotic withinthe gut. This can also occur with other medications patients may bereceiving for unrelated conditions. A further disadvantage of ionexchange resins is the disagreeable lingering taste which is associatedwith oral administration of these compounds.

With respect to methods of diagnosis, one method for detecting C.difficile in a sample is to culture the sample. The disadvantages ofthis method include the length of time required to obtain a result andinterference by non-pathogenic, i.e. non-toxin producing, C. difficilestrains. Other methods involve the use of specific antisera ormonoclonal antibodies. These methods are based on the detection of toxinA or toxin B in clinical samples. U.S. Pats. Nos. 4,863,852 and5,098,826 describe methods for detecting C. difficile toxin A by the useof reagents containing biological receptors for toxin A, including theαGal(1-3)βGal(1-4)βGlcNAc, X and Y antigen oligosaccharide sequences,bound to a support. U.S. Pat. No. 5,635,606 teaches that certainsynthetic oligosaccharide sequences covalently attached to abiocompatible solid support, e.g., Chromosorb p™, may be used to bindtoxin A.

In view of the above, there is a need for an effective treatment forantibiotic associated diarrhea. In particular, a compound is neededwhich can neutralize C. difficile toxin B and/or both C. difficile toxinA and toxin B. A preferred compound would be administered noninvasively,such as orally, in a suitable pharmaceutical formulation.

SUMMARY OF THE INVENTION

The invention provides compositions and methods for the prevention andtreatment of antibiotic associated diarrhea, pseudomembranous colitisand other conditions caused by Clostridium difficile toxin B.

In one aspect, the invention provides a method to bind and remove C.difficile toxin B from a sample suspected of containing said toxin Bcomprising contacting the sample with at least one toxin B bindingoligosaccharide sequence covalently attached to an inert support througha non-peptidyl compatible linker arm under conditions wherein the toxinB is absorbed to the support; and separating the support containing theabsorbed toxin B from the sample.

In another aspect, the invention provides a method to prevent orameliorate one or more conditions mediated by C. difficile toxin B in apatient suffering from or susceptible to said condition, comprisingadministering to the patient an effective amount of a compositioncomprising at least one toxin B binding oligosaccharide sequencecovalently attached to a pharmaceutically acceptable inert supportthrough a non-peptidyl compatible linker arm, wherein saidoligosaccharide sequence binds toxin B, and wherein the composition iscapable of being eliminated from the gastrointestinal tract.

In a further aspect, the invention provides a pharmaceutical compositionuseful in treating or preventing CDAD and related conditions initiatedby C. difficile toxin B, comprising at least one oligosaccharidesequence covalently attached to a pharmaceutically acceptable inertsupport through a non-peptidyl compatible linker arm, wherein saidoligosaccharide sequence binds toxin B, and a pharmaceuticallyacceptable carrier, wherein said composition is capable of beingeliminated from the gastrointestinal tract.

In yet another aspect, the invention provides a method to bind andremove C. difficile toxins A and B from a sample suspected of containingsaid toxins A and B comprising contacting the sample with at least onetoxin A binding oligosaccharide sequence and at least one toxin Bbinding oligosaccharide sequence covalently attached to an inert supportthrough a non-peptidyl compatible linker arm under conditions whereinthe toxins are absorbed to the support; and separating the supportcontaining the absorbed toxins from the sample.

In a still further aspect, the invention provides a method to prevent orameliorate one or more conditions mediated by C. difficile toxins A andB in a patient suffering from or susceptible to said condition,comprising administering to the patient an effective amount of acomposition comprising at least one toxin A binding oligosaccharidesequence and at least one toxin B binding oligosaccharide sequencecovalently attached to a pharmaceutically acceptable inert supportthrough a non-peptidyl compatible linker arm, wherein saidoligosaccharide sequences bind toxins A and B, and wherein thecomposition is capable of being eliminated from the gastrointestinaltract.

In a yet further aspect, the invention provides a pharmaceuticalcomposition useful in treating or preventing CDAD and related conditionsinitiated by C. difficile toxins A and B, comprising at least oneoligosaccharide sequence covalently attached to a pharmaceuticallyacceptable inert support through a non-peptidyl compatible linker arm,wherein said oligosaccharide sequence(s) binds both toxin A and toxin B,and a pharmaceutically acceptable carrier, wherein said composition iscapable of being eliminated from the gastrointestinal tract.

BRIEF DESCRIPTION OF THE DRAWINGS

FIGS. 1A and B illustrate the time and concentration dependentneutralization of C. difficile toxin B activity using SYNSORB 5-128.

FIG. 2 illustrates that SYNSORB 5174, which has both the Cd and theisomaltose oligosaccharide covalently bound by their respective linkers,neutralized both toxin A and B activity.

DETAILED DESCRIPTION OF THE INVENTION

A. Definitions

As used herein the following terms have the following meanings:

The term "antibiotic-associated bacterial diarrhea" refers to thecondition wherein antibiotic therapy disturbs the balance of themicrobial flora of the gut, allowing pathogenic organisms such asClostridium difficile to flourish. These organisms cause diarrhea.Antibiotic-associated bacterial diarrhea includes such conditions asClostridium difficile associated diarrhea (CDAD) and pseudomembranouscolitis (PMC).

The term "biocompatible" refers to chemical inertness with respect tohuman tissues or body fluids.

The terms "compatible linker arm" or "linker arm" refer to a moietywhich serves to space the oligosaccharide structure from thebiocompatible support and which is bifunctional wherein one functionalgroup is capable of binding to a reciprocal functional group of thesupport and the other functional group is capable of binding to areciprocal functional group of the oligosaccharide structure. Compatiblelinker arms preferred in the present invention are non-peptidyl spacerarms. The oligosaccharide may be linked via an 8-methoxycarbonyloctyllinker or via another appropriate non-peptidyl linker, such as aurea-like linker arm of the formula --NH--(CH₂)_(m) --NHC(O)NH--, wherem is an integer of from about 2 to about 10.

The term "oligosaccharide" means saccharides comprising 1 to about 20saccharide moieties. Saccharide derivatives may also be used assaccharide moieties included in the term oligosaccharide [67-69].

The term "pseudomembranous colitis" (PMC), also known aspseudomembranous enterocolitis or enteritis, refers to the inflammationof the mucous membrane of both small and large intestine with theformation and passage of pseudomembranous material (composed of fibrin,mucous, necrotic epithelial cells and leukocytes) in the stools.

The term "support" refers to an inert material to which theoligosaccharide sequences may be bound or immobilized via a compatiblelinker arm. Where use is in vivo, the support will be biocompatible.

The term "SYNSORB" refers to 8-methoxycarbonyloctyl oligosaccharidestructures covalently coupled to Chromosorb P™(Manville Corp., Denver,Colo.) [12], a derivatized silica particle material. Where indicated,the SYNSORB may use a urea-like linker arm rather than the8-methoxycarbonyloctyl linker.

The term "toxin A" refers to an enterotoxin of Clostridium difficilewhich initiates CDAD and related conditions. This toxin has alectin-like activity.

The term "toxin B" refers to a cytotoxin of Clostridium difficile whichcauses destruction of intestinal cells and induces the release ofinflammatory mediators.

For purpose of this application, all sugars are referenced usingconventional three letter nomenclature. All sugars are assumed to be inthe D-form unless otherwise noted, except for fucose, which is in theL-form. Further all sugars are in the pyranose form.

B. Pharmacology

Amino acid sequences in C. difficile toxin A and B that are similar tosequences responsible for oligosaccharide binding in Streptococcalglucan binding proteins have been reported [49-51]. Although, as notedabove, the receptor for toxin B is not known, the oligosaccharidebinding specificity for these glucan binding proteins is for repeatedglucose units linked together as shown below [52]:

    αGlc(1→6)αGlc(1→6)αGlc . . .

The oligosaccharide isomaltotriose (αGlc(1-6)αGlc(1-6)Glc) wasimmobilized by attachment onto Chromosorb P using a linker arm, andtested in toxin B neutralization experiments. The results from theseexperiments are presented graphically in FIGS. 1A and 1B, whereconcentration and time dependent neutralization of C. difficile toxin Bcytotoxic activity using immobilized isomaltotriose SYNSORB (n=3) isshown. Concentration neutralization experiments were performed byincubating immobilized isomaltotriose (10, 20 or 40 mg) with 1 mL oftoxin B for 2 hours at room temperature. The amount of toxin activity ineach sample was measured using Chinese hamster ovary (CHO) cells.

The results are presented as the percent activity remaining relative tocontrol toxin solutions that had not been incubated with SYNSORB. Timedependent neutralization experiments were performed by incubating toxinB with 20 mg samples of immobilized isomaltotriose SYNSORB for 1, 2 and4 h at room temperature. A control incubation (4 h) of toxin B withChromosorb P was included to determine the extent of background bindingto the support. The results are presented as the percent activityremaining relative to control toxin solutions that had not beenincubated with SYNSORB and indicate that toxin B bound to isomaltotrioseSYNSORB in a concentration and time dependent manner. The results alsoindicated that toxin B binds to the support slowly, requiring up to 4hours to achieve significant toxin B binding under these conditions.Further, these data show that oligosaccharides which containa(1-6)-linked repeating units of glucose are effective at binding toxinB and can serve as a therapeutic for C. difficile-mediated diarrhea.

SYNSORBs which incorporate oligosaccharides terminating in glucose orN-acetylglucosamine were also examined for toxin B binding by measuringthe cytotoxic activity of toxin B with or without SYNSORB in CHO cells.Results of these studies are shown in Table 1, where * indicatesSYNSORBs using the urea-like linker arm.

                                      TABLE 1                                     __________________________________________________________________________    Toxin B Neutralization Studies                                                                               Percent                                                                       Neutralization                                 SYNSORB      Oligosaccharide                                                                          Percent                                                                              in Presence                                    Number                                                                              Common Name                                                                          Structure  Neutralization                                                                       of 0.5% BSA                                    __________________________________________________________________________    23    --     βGlc  0       0                                             38    --     αGlc(1-2)βGal                                                                  78 ± 16                                                                          80                                             3-74* maltose                                                                              αGlc(1-4)βGlc                                                                 96 ± 0                                                                            80                                             3-76* cellobiose                                                                           βGlc(1-4)βGlc                                                                  93 ± 5                                                                            80                                             5-128*                                                                              isomaltotriose                                                                       αGlc(1-6)αGlc(1-6)βGlc                                                  96 ± 0                                                                            80                                             179A* isomaltose                                                                           αGlc(1-6)βGlc                                                                 96 ± 9                                                                            N.D.                                           78    chitobiose                                                                           βGlcNAc(1-4)βGlcNAc                                                            93 ± 5                                                                            80                                             __________________________________________________________________________

All SYNSORBS tested except SYNSORB 23 effectively neutralized toxin Bcytotoxicity. By comparison, toxin A did not bind to the SYNSORBs 5-128(isomaltotriose) and 179A (isomaltose). The other SYNSORBs in Table 1were not tested against toxin A. This observation confirms that thereare differences in the binding specificity of toxin A and toxin B, eventhough there is some amino acid homology (60% amino acid homology)between the two toxins. Oligosaccharides which bind toxin A have beenidentified [65-66].

We also utilized a SYNSORB derivative that incorporates two differentoligosaccharide ligands. The ligands selected for the dual labelling ofChromosorb P™ were based on previous results which revealed differentialoligosaccharide binding specificities for toxins A and B. Since theoligosaccharide αGal(1-3)βGal(1-4)βGlc (Cd) binds toxin A but not toxinB, it was selected for use as the toxin A neutralizing component, andwas immobilized onto amino derivatized Chromosorb P using an8-methoxycarbonyl octyl linker arm. Toxin B but not toxin A binds toisomaltose (αGlc(1-6)Glc). Utilizing the amino derivatized Chromosorbthat already incorporated the Cd oligosaccharide, isomaltose wasimmobilized onto the support using the recently developed "Instasorb"linker arm technology as disclosed in PCT/CA97/00851 [70]. The resultingSYNSORB (SYNSORB 5174, which has both oligosaccharides covalently boundby their respective linkers) was then tested for toxin A and B binding.SYNSORB Cd and isomaltose SYNSORB (SYNSORB 179A) were included ascontrols. The results, presented in FIG. 3, show that SYNSORB 5174neutralized both toxin A and B activity. The results also indicate thetoxin neutralizing capacity of SYNSORB 5174 was comparable to SYNSORB Cdand SYNSORB 179A. Thus, a support comprising more than oneoligosaccharide ligand can be used to bind both toxin A and toxin B.

C. Synthesis

Chemical methods for the synthesis of oligosaccharide structures can beaccomplished by methods known in the art. These materials are generallyassembled using suitably protected individual monosaccharides.

The specific methods employed are generally adapted and optimized foreach individual structure to be synthesized. In general, the chemicalsynthesis of all or part of the oligosaccharide glycosides firstinvolves formation of a glycosidic linkage on the anomeric carbon atomof the reducing sugar or monosaccharide. Specifically, an appropriatelyprotected form of a naturally occurring or of a chemically modifiedsaccharide structure (the glycosyl donor) is selectively modified at theanomeric center of the reducing unit so as to introduce a leaving groupcomprising halides, trichloroacetimidate, acetyl, thioglycoside, etc.The donor is then reacted under catalytic conditions well known in theart with an aglycon or an appropriate form of a carbohydrate acceptorwhich possesses one free hydroxyl group at the position where theglycosidic linkage is to be established.

A large variety of aglycon moieties are known in the art and can beattached with the proper configuration to the anomeric center of thereducing unit. Appropriate use of compatible blocking groups, well knownin the art of carbohydrate synthesis, will allow selective modificationof the synthesized structures or the further attachment of additionalsugar units or sugar blocks to the acceptor structures.

After formation of the glycosidic linkage, the saccharide glycoside canbe used to effect coupling of additional saccharide unit(s) orchemically modified at selected positions or, after conventionaldeprotection, used in an enzymatic synthesis. In general, chemicalcoupling of a naturally occurring or chemically modified saccharide unitto the saccharide glycoside is accomplished by employing establishedchemistry well documented in the literature [21-37].

The supports to which the oligosaccharide structures of the presentinvention are bound or immobilized include a wide variety ofbiocompatible materials known in the art. Water soluble biocompatiblepolymers such as hydrogels, carboxymethyl celluloses, syntheticpolymers, and the like are particularly preferred. In particular, thesesupports are useful for delivery to the gut, especially prolongeddelivery. Useful supports are non-absorbable, that is to say that theymay be soluble or insoluble, so long as they are not absorbed by thebody.

Solid supports are particularly useful for certain applications. Suchsolid supports to which the oligosaccharide structures of the presentinvention are bound may be in the form of sheets or particles. A largevariety of biocompatible solid support materials are known in the art.Examples thereof are silica, synthetic silicates such as porous glass,biogenic silicates such as diatomaceous earth, silicate-containingminerals such as kaolinite, and synthetic polymers such as polystyrene,polypropylene, and polysaccharides. Preferably the solid supports have aparticle size of from about 10 to 500 microns for in vivo use. Inparticular, particle sizes of 100 to 200 microns are preferred.

The oligosaccharide structure(s) is covalently bound or noncovalently(passively) adsorbed onto the support so as to be immobilized. Thecovalent bonding may be via reaction between functional groups on thesupport and the compatible linker arm of the oligosaccharide structure.It has unexpectedly been found that attachment of the oligosaccharidestructure to the biocompatible support through a compatible linking armprovides a product which, notwithstanding the support, effectivelyremoves toxin. Linking moieties that are used in indirect bonding arepreferably organic bifunctional molecules of appropriate length (atleast one carbon atom) which serve simply to distance theoligosaccharide structure from the surface of the support.

The compositions of this invention are preferably represented by theformula:

    (OLIGOSACCHARIDE--Y--R).sub.n --SUPPORT

where OLIGOSACCHARIDE represents an oligosaccharide group of at least 1sugar unit which group binds to toxin B or toxins A and B, Y is oxygen,sulfur or nitrogen, R is an aglycon linking arm of at least 1 carbonatom, SUPPORT is as defined above, and n is an integer greater than orequal to 1. Oligosaccharide sequences containing about 2 to 10saccharide units may be used. Sequences with about 2 to 4 saccharideunits are preferred. In some instances, more than one oligosaccharidegroup may be linked to the support, e.g., one oligosaccharide groupwhich binds toxin B and another which binds toxin A, to provide acomposition which binds to more than one toxin moiety.

Numerous aglycon linking arms are known in the art. For example, alinking arm comprising a para-nitrophenyl group (i.e., --OC₆ H₄ pNO₂)has been disclosed [38]. At the appropriate time during synthesis, thenitro group is reduced to an amino group which can be protected asN-trifluoroacetamido. Prior to coupling to a support, thetrifluoroacetamido group is removed thereby unmasking the amino group.

A linking arm containing sulfur has been disclosed [39]. Specifically,the linking arm is derived from a 2-bromoethyl group which, in asubstitution reaction with thionucleophiles, has been shown to lead tolinking arms possessing a variety of terminal functional groups such as,--OCH₂ CH₂ SCH₂ CO₂ CH₃ and --OCH₂ CH₂ SC₆ H.sub. 4--pNH₂. Theseterminal functional groups permit reaction to complementary functionalgroups on the support, thereby forming a covalent linkage to thesupport. Such reactions are well known in the art.

A 6-trifluoroacetamido-hexyl linking arm, (--O--(CH₂)₆ --NHCOCF₃) hasbeen disclosed [40] in which the trifluoroacetamido protecting group canbe removed, unmasking the primary amino group used for coupling.

Other exemplifications of known linking arms include the7-methoxycarbonyl-3,6,dioxaheptyl linking arm [41] (--OCH₂ --CH₂)₂ OCH₂CO₂ CH₃); the 2-(4-methoxycarbonylbutan-carboxamido) ethyl [42] (--OCH₂CH₂ NHC(O)(CH₂)₄ CO₂ CH₃); the allyl linking arm [43] (--OCH₂ CH=CH₂)which, by radical co-polymerization with an appropriate monomer, leadsto co-polymers; other allyl linking arms [44] are known (--O(CH₂ CH₂ O)₂CH₂ CH=CH₂). Additionally, allyl linking arms can be derivatized in thepresence of 2-aminoethanethiol [45] to provide for a linking arm --OCH₂CH₂ CH₂ SCH₂ CH₂ NH₂. Other suitable linking arms have also beendisclosed [21-23, 25, 26]. The particular linking employed to covalentlyattach the oligosaccharide group to the support is not critical.

Preferably, the aglycon linking arm is a hydrophobic group and mostpreferably, the aglycon linking arm is a hydrophobic group selected fromthe group consisting of ##STR1## --(CH₂)₅ OCH₂ CH₂ CH₂ --, --(CH₂)₈ CH₂O--and --NH--(CH₂)_(m) --NHC(O)NH--, where m is an integer of from about2 to about 10. ##STR2##

Where R¹ is selected from the group consisting of a covalent bond and adivalent hydrocarbylene group having from 1 to about 20 carbon atoms; R²is a divalent hydrocarbylene group of from 2 to about 20 carbon atoms;each X' is independently selected from the group consisting of --O-- and>NR⁴ wherein each R⁴ is independently selected from hydrogen, --R² NH,or --R² NR³ Z wherein R² is as defined above; R³ is selected from thegroup consisting of hydrogen and --C(O)R⁵ wherein R⁵ is hydrocarbyl offrom 1 to about 20 carbon atoms; W is selected from O or S; X isselected from the group consisting of --NH--, --O-- and --S--; Y isselected from the group consisting of --NH--, --O-- and --S--; and p isan integer from 0 to 50 or more, wherein the total number of atomsseparating the support from the toxin-binding oligosaccharide is atleast 8.

We have found that synthetic oligosaccharide sequences covalentlyattached to a biocompatible support, e.g., Chromosorb P™ (SYNSORB) maybe used to bind toxin B. These compositions are useful to treat orprevent CDAD, PMC and other conditions associated with C. difficileinfection. When a solid support is to be used, SYNSORB is particularlypreferred for these compositions because it is non-toxic and resistantto mechanical and chemical degradation.

In studies using rats (a widely accepted model for preclinical studies,since they are predictive of human response), SYNSORBs have been foundto pass unaffected through the rat gastrointestinal tract. They werefound to be eliminated completely and rapidly (99% eliminated in 72hours) following oral administration. Additionally, the high density ofoligosaccharide moieties on SYNSORBs is particularly useful for bindingtoxins which have carbohydrate binding affinity. For example, toxin A isthought to possess multiple oligosaccharide binding sites [11].

Non-peptidyl linking arms are preferred for use as the compatiblelinking arms of the present invention. The use of glycopeptides is notdesirable because glycopeptides contain several, often different,oligosaccharides linked to the same protein. Glycopeptides are alsodifficult to obtain in large amounts and require expensive and tediouspurification. Likewise, the use of BSA or HSA conjugates is notdesirable due to questionable stability in the gastrointestinal tractwhen given orally.

Covalent attachment of an oligosaccharide group containing a toxin Bbinding unit through a non-peptidyl spacer arm to an inert supportpermits efficient binding and removal of toxin B or toxins A and B froma sample to be analyzed for the presence of toxin B (or toxins A and B)or from the intestine of a patient suffering from or susceptible toCDAD, PMIIC or another condition associated with C. difficile infection.When the oligosaccharide is synthesized with this compatible linker armattached (in non-derivatized form), highly pure compositions may beachieved which can be coupled to various supports.

D. Pharmaceutical Compositions

The methods of this invention are achieved by using pharmaceuticalcompositions comprising one or more oligosaccharide structures whichbind toxin B attached to a support.

When used for oral administration, which is preferred, thesecompositions may be formulated in a variety of ways. It will preferablybe in liquid or semisolid form. Compositions including a liquidpharmaceutically inert carrier such as water may be considered for oraladministration. Other pharmaceutically compatible liquids or semisolids,may also be used. The use of such liquids and semisolids is well knownto those of skill in the art. (See, e.g., Remington's PharmaceuticalSciences, 18th edition, 1990.)

Compositions which may be mixed with liquid or semisolid foods such asenteral nutritional formulas, applesauce, ice cream or pudding may alsobe preferred. Formulations, such as SYNSORBs, which do not have adisagreeable taste or aftertaste are preferred. A nasogastric tube mayalso be used to deliver the compositions directly into the stomach.

Solid compositions may also be used, and may optionally and convenientlybe used in formulations containing a pharmaceutically inert carrier,including conventional solid carriers such as lactose, starch, dextrinor magnesium stearate, which are conveniently presented in tablet orcapsule form. The (OLIGOSACCHARIDE--Y--R)_(n) --SUPPORT compositionitself may also be used without the addition of inert pharmaceuticalcarriers, particularly for use in capsule form.

Doses are selected to provide neutralization and elimination of toxin Bfound in the gut of effected or at risk subjects. Useful doses are fromabout 0.25 to 1.25 micromoles of oligosaccharide/kg body weight/day,preferably about 0.5 to 1.0 micromoles of oligosaccharide/kg bodyweight/day. Using SYNSORB compositions, this means about 0.5 to 1.0 gramSYNSORB/kg body weight/day, which gives a concentration of SYNSORB inthe gut of about 20 mg/ml. For subjects with clinical symptoms,administration is expected to be 3 or 4 times daily, for a period of oneweek or until clinical symptoms are resolved. For at risk subjects,prolonged prophylactic administration, e.g., in enteral nutritionalformulas, is indicated. The dose level and schedule of administrationmay vary depending on the particular oligosaccharide structure used andsuch factors as the age and condition of the subject.

As discussed previously, oral administration is preferred, butformulations may also be considered for other means of administrationsuch as per rectum. The usefulness of these formulations may depend onthe particular composition used and the particular subject receiving thetreatment. These formulations may contain a liquid carrier that may beoily, aqueous, emulsified or contain certain solvents suitable to themode of administration.

Compositions may be formulated in unit dose form, or in multiple orsubunit doses. For the expected doses set forth previously, orallyadministered liquid compositions should preferably contain about 1micromole oligosaccharide/ml.

E. Methodology

We have found that C. difficile toxin B may be neutralized by certainoligosaccharide sequences which bind the toxin. In particular, syntheticoligosaccharides covalently attached to supports via non-peptidylcompatible linker arms have been found to neutralize toxin B or toxins Aand B effectively. Examples of such compositions are certain SYNSORBs,which neutralize the activity of toxin B or toxins A and B.

We have tested the ability of several oligosaccharide sequences attachedto Chromosorb P via 8-methoxylcarbonyloctyl (MCO) or urea-like spacerarms to neutralize toxin B or toxins A and B. The oligosaccharidesequences attached to supports useful in the present invention are thosewhich bind toxin B and, in some cases, both toxins A and B.

The binding affinity of an oligosaccharide to toxin B is readilydetectable by a simple in vitro test, as for example, set forth inExample 3 below. For the purposes of this invention, oligosaccharidesequences attached to supports which bind toxin B means thosecompositions which reduce cytotoxicity in CHO cell assays by at least50%.

Several different oligosaccharide sequences attached to supports viacompatible arms have been found to have the ability to neutralize toxinB (and in some cases, toxin A and B) activity. These sequences, andothers that also bind toxin B, may be used to treat or prevent CDAD, PMCand other conditions associated with C. difficile infection. The optimaltime for complete removal of toxin B activity was found to be about 4hours at 37° C., using a concentration of SYNSORB of 20 mg in 1 mlsample. Since each gram of SYNSORB contains approximately 0.25 to 1.0micromoles oligosaccharide, the total amount of oligosaccharide to begiven in a daily dose would range from 7.5 to 30 micromoles, using a gutvolume of four liters.

Treatment or prevention of CDAD, PMC or other conditions associated withC. difficile infection may be accomplished by oral administration ofcompositions containing oligosaccharide sequences covalently bound to asupport via a compatible linker arm (e.g., SYNSORBs). For example,SYNSORBs have been found to pass through the stomach of rats intact.This means that they are intact when they contact toxin B in theintestinal tract. Subsequent elimination of intact SYNSORB with toxin Bbound to it results in elimination of toxin B from the patient.

Oligosaccharide sequences covalently attached via compatible linker armsto a support, e.g., SYNSORBs, are useful to treat individuals who sufferfrom multiple episodes of diarrhea. Upon initial reoccurrence ofdiarrhea, patients would be treated with SYNSORB to remove toxin B orboth toxin A and toxin B from the intestine. The removal of toxin Aprevents the initial tissue damage to the intestinal lining, which leadsto prevention or reduction of diarrhea. Removal of toxin B prevents thecytotoxicity of this toxin to the intestinal and colonic cells, whichalso leads to prevention or reduction of diarrhea. No further treatmentwith antibiotics need be given, allowing the re-establishment of thenormal intestinal microflora within the gut. The advantage of suchtreatment is that it does not affect the recolonization of theintestinal tract by normal microflora. Treatment until discontinuance ofdiarrhea would allow complete recovery.

In addition to its usefulness in patients suffering from recurringdiarrhea, treatment with oligosaccharide sequences covalently attachedvia compatible linker arms to supports, e.g., SYNSORBs, may be used totreat all individuals who suffer from or are prone to develop CDAD, PMCor other conditions associated with C. difficile infection. The use ofthe oligosaccharide-support compositions of the present invention incombination with antibiotic therapy will be able to reduce the diarrheamore effectively, leading to more rapid recovery.

Toxin B and/or toxin A may be measured directly on the surface of theoligosaccharide-containing support using any suitable detection system.For example, radioactive, biotinylated or fluorescently labelledmonoclonal or polyclonal antibodies specific for the toxin may be usedto determine the amount of toxin bound to the support. A wide variety ofprotocols for detection of formation of specific binding complexesanalogous to standard immunoassay techniques is well known in the art.

EXAMPLES

The following methods were used to perform the studies in the Examplesthat follow. Terms and abbreviations are consistent with those incurrent use in this art.

1. Toxin Purification

Toxins A and B were isolated from a toxin producing strain of C.difficile (ATCC 43255, VPI strain 10463) using slight modifications ofthe method of Sullivan et al. as described previously [2,21]. The toxinB fraction was devoid of toxin A activity, as determined by theinability of the toxin containing solution to hemagglutinate rabbiterythrocytes.

2. Toxin A Hemagglutination Assays Using Rabbit Erythrocytes

Fresh rabbit erythrocytes were washed once in Tris buffered saline (TBS,pH 7.4) and resuspended at a concentration of 4% (vol/vol) in TBS.Serial two-fold dilutions (50 μL) of toxin A solutions were made in TBSin U-shaped microtitre wells. An equal volume (50 μL) of rabbiterythrocytes was then added to each well and the microtitre plate wasmixed gently. Toxin A hemagglutination assays were incubated at 4° C.for 4 h. The hemagglutination titre was then assessed visually. Allassays were done in duplicate.

3. Assay of Toxin B Activity Using Chinese Hamster Ovary Cells

Chinese hamster ovary (CHO) cells were maintained in Hams F12 mediasupplemented with 10% fetal bovine serum in an atmosphere of 5% CO₂ at37° C. Samples to be tested for toxin B activity were diluted 1:10 inHams media and filter sterilized through 0.22 micron syringe filters.Samples were serial 3-fold diluted in media and 1 00 μL of each dilutionwas added to wells with confluent monolayers of CHO cells and incubatedfor 24 h at 37° C. / 5%CO₂.

Each sample was analyzed two times. Cytotoxic effects were recordedafter 24 h incubation by comparing test sample wells with control wellsthat did not contain toxin B. After 24 h, the cells were fixed with 95%methanol and stained with Geimsa stain. Samples from neutralizationexperiments were treated in an analogous fashion. The percentneutralization was determined by comparing the end point dilutions ofsamples with and without SYNSORB treatment.

4. Toxin A and B Neutralization Assays

PBS solutions with or without 0.5% BSA containing purified toxin Band/or A (0.5 mL) were added to SYNSORBs (10 mg) in 0.5 mLmicrocentrifuge tubes and incubated at room temperature for 1 h on anend-over-end rotator. After incubation, the SYNSORB was allowed tosettle to the bottom of the tubes and the supernatants were carefullyremoved. Serial two-fold dilutions of the supernatants were prepared inTris buffered saline (TBS) and the end point titers in thehemagglutination or CHO cell assays was determined as described above.The percent binding of either toxin B and/or toxin A was calculatedrelative to the end-point titers of toxin solutions incubated with noSYNSORB or with Chromosorb P containing only the non-peptidyl linkerarm.

Example 1

Determining Conditions for Toxin B Binding to Isomaltotriose SYNSORB

The conditions required for toxin B binding were determined byincubating 20 mg samples of isomaltotriose SYNSORB (5-128) or Chromosorbwith 1 mL of a purified toxin B solution in 1.5 mL microcentrifuge tubesfor 1, 2 and 4 h at room temperature on an end-over-end rotator. Controltubes containing toxin B solution but no SYNSORB or Chromosorb wereincubated at the same time. Determination of the optimal amount ofisomaltotriose SYNSORB required for maximum toxin B neutralization wasperformed by incubating immobilized isomaltotriose (10, 20 or 40 mg)with 1 mL of toxin B for 2 hours at room temperature. The amount oftoxin activity in each sample was measured using CHO cells. Afterincubation, the SYNSORB was allowed to settle to the bottom of the tubesand the supernatants were carefully removed. Serial five-fold dilutionsof the supernatants were prepared and the cytotoxic end point determinedas described above. Each experiment was done in at least duplicate. Theextent of reduction in the end point in the presence of SYNSORB wasdetermined by comparing with controls in which SYNSORB was not added.The results of these experiments are presented in FIGS. 1A and 1B, andshow that SYNSORB 5174 was effective to neutralize toxin B activity.

Example 2

Screening of Oligosaccharides for Toxin B Neutralization

Solutions containing purified toxin B (1 mL) were added to variousSYNSORBs listed in Table 1 (20 mg) containing different oligosaccharidesequences in 1.5 mL microcentrifuge tubes and incubated at roomtemperature for 4 h on an end-over-end rotator. The amount ofneutralization in each sample was determined by comparing the cytotoxicend point titres of CHO cell assays from samples with and withoutSYNSORB.

As shown in Table 1, all of the oligosaccharides tested except βGlceffectively neutralized toxin B cytotoxicity. Thus, the oligosaccharidesαGlc(1-2)βGal, αGlc(1-4)βGlc (maltose), βGlc(1-4)βGlc(cellobiose),αGlc(1-6)αGlc(1-6)αGlc (isomaltotriose), αGlc(1≠6)αGlc (isomaltose) andβGlcNAc(1-4)βGlcNAc (chitobiose) bound toxin B.

Example 3

Toxin A Neutralization Assays Using Isomaltose and IsomaltotrioseSYNSORBs

Solutions containing purified toxin A (0.5 mL) were added to isomaltoseor isomaltotriose SYNSORBs (10 or 20 mg) in 0.5 mL microcentrifuge tubesand incubated at either 4° C. or room temperature for 1 h on anend-over-end rotator. After incubation, the SYNSORB was allowed tosettle to the bottom of the tubes and the supernatants were carefullyremoved. Serial two-fold dilutions of the supernatants were prepared inTris buffered saline (TBS) and the hemagglutination end point determinedas described above. The extent of reduction in the end point in thepresence of either SYNSORB was determined by comparing with controls inwhich SYNSORB was not added. We did not detect any toxin A binding toeither isomaltose or isomaltotriose SYNSORB, indicating that toxin A hasdifferent binding specificity from toxin B.

Example 4

Neutralization of Both Toxin A and Toxin B

Neutralization experiments of C. difficile toxin A hemagglutinating andtoxin B cytotoxic activity were performed using a "dual-labelled"SYNSORB, i.e., SYNSORB 5174 which has both the Cd oligosaccharide(αGal(1-3)βGal(1-4)βGlc) and isomaltose, each attached by its respectivelinker (n=2). Neutralization experiments were done by incubating eitherSYNSORB 5174, 179A (isomaltose) or Cd at a concentration of 20 mgl mLwith toxin A for 1 h or toxin B for 4 hours at room temperature. Theamount of toxin activity in each sample was measured using CHO cells orrabbit erythrocytes. The results are presented as the percent activityremaining relative to control toxin solutions that had not beenincubated with SYNSORB.

The results, presented in FIG. 2, show that SYNSORB 5174 neutralizedboth toxin A and B activity. The results also indicate the toxinneutralizing capacity of SYNSORB 5174 was comparable to SYNSORB Cd andSYNSORB 179A. Thus, a support comprising more than one oligosaccharideligand can be used to bind both toxin A and toxin B.

What is claimed is:
 1. A pharmaceutical composition useful to bind C.difficile toxin B in a subject, which composition comprises:a) at leastone oligosaccharide sequence covalently attached to a pharmaceuticallyacceptable inert support through a non-peptidyl compatible linker arm,wherein said oligosaccharide sequence binds toxin B; and b) apharmaceutically acceptable carrier, wherein said composition is capableof being eliminated from the gastrointestinal tract, and wherein saidoligosaccharide sequence is αGlc(1-6)αGlc(1-6)βGlc and said linker armis --NH--(CH₂)_(m) --NHC(O)NH--, where m is an integer of from about 2to about
 10. 2. A pharmaceutical composition useful to bind C. difficiletoxin B in a subject, which composition comprises:a) at least oneoligosaccharide sequence covalently attached to a pharmaceuticallyacceptable inert support tough a non-peptidyl compatible linker arm,wherein said oligosaccharide sequence binds toxin B; and b) apharmaceutically acceptable carrier, wherein said composition is capableof being eliminated from the gastrointestinal tract, and wherein saidoligosaccharide sequence is αGlc(1-6)βGlc and said linker arm is--NH--(CH₂)_(m) --NHC(O)NH--, where m is an integer of from about 2 toabout
 10. 3. A method to bind toxin B in a subject, which methodcomprises administering to said subject a composition comprising theoligosaccharide sequence αGlc(1-6)αGlc(1-6)βGlc covalently attached to apharmaceutically acceptable inert support through a linker arm which is--NH--(CH₂)_(m) --NHC(O)NH--, where m is an integer of from about 2 toabout 10, wherein said composition contacts and binds toxin B, andwherein said composition is capable of being eliminated from thegastrointestinal tract.
 4. A method to bind toxin B in a subject, whichmethod comprises administering to said subject a composition comprisingthe oligosaccharide sequence αGlc(1-6)βGlc covalently attached to apharmaceutically acceptable inert support through a linker arm which is--NH--(CH₂)_(m) --NHC(O)NH--, where m is an integer of from about 2 toabout 10, wherein said composition contacts and binds toxin B, andwherein said composition is capable of being eliminated from thegastrointestinal tract.
 5. A pharmaceutical composition useful to bindC. difficile toxin B in a subject, which composition comprises:a) atleast one oligosaccharide sequence covalently attached to apharmaceutically acceptable inert support through a non-peptidylcompatible linker arm, wherein said oligosaccharide sequence binds toxinB; and b) a pharmaceutically acceptable carrier, wherein saidcomposition is capable of being eliminated from the gastrointestinaltract, and wherein said oligosaccharide sequence isαGlc(1-6)αGlc(1-6)βGlc and said linker arm is ##STR3## where R' isselected from the group consisting of a covalent bond and a divalenthydrocarbylene group having from 1 to about 20 carbon atoms; R² is adivalent hydrocarbylene group of from 2 to about 20 carbon atoms; eachX' is independently selected from the group consisting of --O-- and >NR⁴wherein each R⁴ is independently selected from hydrogen, --R² NH₂ or--R2NR³ Z wherein R² is as defined above; R³ is selected from the groupconsisting of hydrogen and --C(O)R⁵ wherein R⁵ is hydrocarbyl of from 1to about 20 carbon atoms; W is selected from O or S; X is selected fromthe group consisting of --NH--, --O-- and --S--; Y is selected from thegroup consisting of --NH--, --O-- and --S--; and p is an integer of from0 to 50, wherein the total number of atoms separating the support fromthe toxin-binding oligosaccharide is at least
 8. 6. A pharmaceuticalcomposition useful to bind C. difficile toxin B in a subject, whichcomposition comprises:a) at least one oligosaccharide sequencecovalently attached to a pharmaceutically acceptable inert supportthrough a non-peptidyl compatible linker arm, wherein saidoligosaccharide sequence binds toxin B; and b) a pharmaceuticallyacceptable carrier, wherein said composition is capable of beingeliminated from the gastrointestinal tract, and wherein saidoligosaccharide sequence is αGlc(1-6)αGlc(1-6)βGlc and said linker armis ##STR4## where R¹ is selected from the group consisting of a covalentbond and a divalent hydrocarbylene group having from 1 to about 20carbon atoms; R² is a divalent hydrocarbylene group of from 2 to about20 carbon atoms; each X' is independently selected from the groupconsisting of --O-- and >NR⁴ wherein each R⁴ is independently selectedfrom hydrogen, --R² NH₂ or --R² NR³ Z wherein R² is as defined above; R³is selected from the group consisting of hydrogen and --C(O)R⁵ whereinR⁵ is hydrocarbyl of from 1 to about 20 carbon atoms; W is selected fromO or S; X is selected from the group consisting of --NH--, --O-- and--S--; Y is selected from the group consisting of --NH--, --O--and--S--; and p is an integer of from 0 to 50, wherein the total number ofatoms separating the support from the toxin-binding oligosaccharide isat least
 8. 7. A method to bind toxin B in a subject, which methodcomprises administering to said subject a composition comprising theoligosaccharide sequence αGlc(1-6)αGlc(1-6)βGlc covalently attached to apharmaceutically acceptable inert support through a linker arm which isαGlc(1-6)αGlc(1-6)βGlc and said linker arm is ##STR5## where R¹ isselected from the group consisting of a covalent bond and a divalenthydrocarbylene group having from 1 to about 20 carbon atoms; R² is adivalent hydrocarbylene group of from 2 to about 20 carbon atoms; eachX' is independently selected from the group consisting of --O-- and >NR⁴wherein each R⁴ is independently selected from hydrogen, --R₂ NH₂ or--R² NR³ Z wherein R² is as defined above; R³ is selected from the groupconsisting of hydrogen and --C(O)R⁵ wherein R⁵ is hydrocarbyl of from 1to about 20 carbon atoms; W is selected from O or S; X is selected fromthe group consisting of --NH--, --O-- and --S--; Y is selected from thegroup consisting of --NH--, --O-- and --S--; and p is an integer of from0 to 50, wherein the total number of atoms separating the support fromthe toxin-binding oligosaccharide is at least 8,wherein said compositioncontacts and binds toxin B, and wherein said composition is capable ofbeing eliminated from the gastrointestinal tract.
 8. A method to bindtoxin B in a subject, which method comprises administering to saidsubject a composition comprising the oligosaccharide sequenceαGlc(1-6)βGlc covalently attached to a pharmaceutically acceptable inertsupport through a linker arm which is αGlc(1-6)αGlc(1-6)βGlc and saidlinker arm is ##STR6## where R¹ is selected from the group consisting ofa covalent bond and a divalent hydrocarbylene group having from 1 toabout 20 carbon atoms; R² is a divalent hydrocarbylene group of from 2to about 20 carbon atoms; each X' is independently selected from thegroup consisting of --O-- and >NR⁴ wherein each R⁴ is independentlyselected from hydrogen, --R² NH₂ or --R² NR³ Z wherein R² is as definedabove; R³ is selected from the group consisting of hydrogen and --C(O)R⁵wherein R⁵ is hydrocarbyl of from 1 to about 20 carbon atoms; W isselected from O or S; X is selected from the group consisting of --NH--,--O-- and --S--; Y is selected from the group consisting of --NH--,--O-- and --S--; and p is an integer of from 0 to 50, wherein the totalnumber of atoms separating the support from the toxin-bindingoligosaccharide is at least 8,wherein said composition contacts andbinds toxin B, and wherein said composition is capable of beingeliminated from the gastrointestinal tract.